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2020;77:951-969.e959

(2000) controlled for at least smoking and body mass index

These cascades involve the activation of NF-B and MAPK pathways, upregulation of inflammatory cytokines, and overproduction of reactive oxygen species (ROS), collectively accelerating cellular senescence and tissue degradation 8,9,10,11,12

AB staining identified sperm cells with abnormal chromatin packaging (dark blue nuclei), whereas AO staining distinguished between spermatozoa with normal DNA (green) and damaged DNA (orange/red) 99