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Do not blow on them

doi: 10.1074/jbc.M302227200

doi: 10.1007/s00204-024-03696-4, 212

Note that peptide bond, an amide bond, is strong and does not have free rotation around it because of the partial double bond character (see resonance structure)

Next, after 3 washings with PBS-Tween 0.1% and 2 washings with the B/W buffer for 5 minutes at RT, 8 pmol of ssDNA pool from the rounds of selection were added to each dot and incubated for 1 hour at 22 C, then washed 3 times with the B/W buffer