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The experiments first assessed the direct impact of DSIP on freshly isolated rat brain mitochondria and brain homogenates under normal (non-hypoxic) conditions using polarographic measurements of oxygen consumption

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Rather than relying solely on appetite suppression, emerging therapies aim to address the complex hormonal systems governing energy balance and metabolism

Then, the cells were trypsinised, fixed with 4% PFA, permeabilised and labelled with Alexa Fluor 647 picolyl azide

Consistency with the protocol matters more than genetic luck in determining outcomes