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Wash the column with 10 - 15 bed volumes of Wash buffer until A280 is stable at the flow-rate of 1 mL per minute

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doi: 10.1016/j.mehy.2004.03.021

5-Azacitidine is modified by nucleotide reductase and then phosphorylated by uridine cytidine kinase to form a triphosphate, 5-azacitidine triphosphate, which is integrated into the DNA sequence as a cytosine analog during DNA replication, thereby inhibiting DNA cytosine methylation (Rius et al., 2009)