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Lancet Neurology 19, 179194

The appropriate anti-human/mouse immunoglobulin G (IgG) secondary antibodies conjugated to horseradish peroxidase (Sigma, USA) were then added at a final dilution of 1:1,000 in M-TBS/T and incubated at room temperature for 1 h

The elimination rate constant is used to calculate the drugs half-life (T ), or the time required for drug concentration to decrease by one half (Figure 4-9)

Recognizing the nuances of how oxidative stress induces cell death is crucial for developing targeted therapeutic strategies to combat conditions associated with excessive ROS production or impaired antioxidant defenses