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DE induction was performed using the STEMdiff Definitive Endoderm Kit from STEMCELL Technologies according to the manufacturer's protocol with small modifications

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However, additional equipment is required for LSD screening methods, as they are not compatible with the existing method for amino acids and acylcarnitines.Furthermore, FIA does not adequately prevent contaminants from the work-up of enzymatic products from interfering in the LSD assays, which limits the efficiency of the ion source

Pathway-Level Metabolic Research : Examining how AOD 9604 affects pathways linked to fat and energy regulation, like -adrenergic and AMPK pathways, to understand how this peptide influences energy use in cells

Kim JK, Park SU