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Cells were resuspended in 250 l relevant culture medium supplemented with rho-kinase inhibitor Y-27632 (overnight) and optionally long term with 5 nM scTS2/16

The simplicity with which GST-fusion proteins can be purified to homogeneity in a one-step affinity chromatography using glutathione immobilized to a matrix (or support) and glutathione itself serving as the eluant further lends support to the choice of GST

If patients develop skin-related toxicities, the nurse should grade the toxicity, provide appropriate supportive care interventions, and work as part of the team for management

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These tools have provided unprecedented insights into the molecular pathways affected by lithium and the potential modulation of glutathione synthesis