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After 24 h, cells were lysed and subjected to luciferase activity using a Luc-Pair Duo-Luciferase HS Assay Kit (GeneCopoeia, MD, USA) according to the manufacturers instructions

With a p K a of ~8.5, the cysteine thiol is deprotonated at higher pH values 32 , allowing it to coordinate FeS clusters

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For instance, TMAO, a metabolite produced by gut bacteria from dietary choline and carnitine, is closely associated with adverse cardiovascular outcomes when its plasma levels are elevated ( Shigella , can promote systemic inflammation, thereby increasing the risk of CVD ( Given the complex interactions between the gut microbiota and drugs, as well as the influence of the gut microbiota on CVD, the gut microbiota also plays a crucial role in shaping the therapeutic effects and side effects of cardiovascular drugs

Through this mechanism, SS peptides could improve muscle mass and delay the progression of muscle atrophy