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The assay involved spiking known concentrations of HO into the milk sample, followed by detection using spectrophotometry and electrochemical methods.The recovery percentage was calculated, yielding a recovery of approximately 95% (see SI Figure S6)

Furthermore, the culture model significantly influences the cellular response in hyperosmotic environments: AD-MSCs showed stronger tolerance in monolayer culture, but their tolerance to hyperosmotic environments was significantly reduced in 3D suspension or hydrogel cultures (Potocar et al., 2016)

We chose to make all transport rates linear and adjusted them so that the measured APAP, APAP-S, APAP-G, and NAPQI-GSH concentrations in the plasma and the urine were as measured in the literature[4, 6, 11, 3032]