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An experiment using human peripheral blood macrophages (M) showed that the intracellular GSH content was dose-dependently increased by treatment of M with cystine, and was increased further by the addition of glutamate (Rimaniol et al

Treatment of cells Hepatoma HepG2 cells were maintained in DMEM (GE Healthcare, Buckinghamshire, UK) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin at 37 C in 5% CO 2

This is because we know the body can utilize up to 90 grams of carbohydrates per hour

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Under specific conditions, astrocytes can also release neurotransmitters like D-serine into the synaptic cleft, thereby regulating the function of neuronal N-methyl-D-aspartate (NMDA) receptors and influencing the strength and direction of neurotransmission (Sacks et al., 2018)