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Randomized participants identified their stronger leg and a micro-needle muscle biopsy was performed on the weaker leg via the Vastus Lateralis by an experienced physician or trained delegate [27]

On the other hand, the experiment was conducted in accordance with the ARRIVE (Animal Research: Reporting of in Vivo Experiments) guidelines [63] for the care and use of research animals Consent for publication NA Competing interests The authors declare that they have no conflict of interest

Nutrition and branched-chain amino acids Malnutrition can lead to a paradoxical increase in ammonia and decreased survival by influencing protein turnover [89], increasing susceptibility to infections, impairing immunocompetence [90] and inducing malabsorption [91]

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