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Reduced serum free carnitine as well as reduced levels of 3-keto-VPA, the main metabolite of -oxidation of VPA, was first reported in 1982 by Bohles and coworkers [53] in a 3-year-old girl who developed acute liver disease with typical features of Reye's syndrome after treatment with VPA for 6 months

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The cells were washed with PBS three times (5 min/wash) and observed under a fluorescence microscope (20, Olympus, Japan)

The anti-inflammatory effects of Omega-3 fatty acids, the improvement of neurotransmitter function, and the promoting effects on brain development may be its potential therapeutic mechanisms

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