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OCT embedded brains were cut into 10 m sections, the sections were rinsed three times with PBS, blocked for 1 h in 8% bovine serum albumin (BSA) dissolved in 0.01 M phosphate buffer (PB) containing 0.5% Triton-X100

Catalpol also promotes the uncoupling of Galectin-3 and CD146 molecular complexes induced by AGEs, improving endothelial damage in hepatic sinusoids and reducing the release of monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1)

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Then, the membranes were washed and incubated with Goat Anti-Rabbit IgG (H + L) HRP (Affinity, S0001) or Goat Anti-Mouse IgG HRP (Affinity, S0002) for 1 h