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With age, Ca decreases with a compensatory increase in PTH

Immunoblotting Liver tissues (human/mouse) were homogenized in ice-cold RIPA lysis buffer (Beyotime, P0013B, supplemented with 1 protease/phosphatase inhibitor cocktail, Roche 05892791001) using a Precellys 24 homogenizer (3 30 s pulses at 6500 rpm), while primary hepatocytes and HepG2 cells were lysed in SDS buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol, 4% -mercaptoethanol, 100 mM DTT, 0.01% bromophenol blue) with brief sonication (Qsonica Q700, 20% amplitude, 3 5 s pulses)

Cammarata PR, Xu GT, Huang L et al: Inducible expression of Na+/myo-inositol cotransporter mRNA in anterior epithelium of bovine lens: Affiliation with hypertonicity and cell proliferation

Eventually this results in difficulty speaking, swallowing, and breathing and often leads to death within a few years after diagnosis

L.DuijstS.RuijterJ