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8 and Wang, et al

The neurons were cultured in Neurobasal medium (Gibco) supplemented with 2% B27 (Gibco), 2 mM Glutamax (Gibco) and 50 U/mL penicillin/ streptomycin (Invitrogen) for 2 days in vitro (2DIV) after being treated with specific reagents

NAD + is concentrated in the mitochondria, with lower concentrations in the nucleus and cytoplasm 76,77

En condiciones de estrs oxidativo severo, la habilidad de la clula para reducir GSSG a GSH se encuentra superada, tendiendo entonces a la acumulacin de GSSG

Nat Commun 8:15965 Bott AJ, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang YP, Yu X, Bhatt V, Chiles E, Zhong H et al (2019) Glutamine anabolism plays a critical role in pancreatic cancer by coupling carbon and nitrogen metabolism