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Co-immunoprecipitation (Co-IP) assay Cells after indicated treatments were lysed in IP buffer (50 mM Tris-HCl, pH 7.5, 0.5% NP-40 (MF430, Mei5bio), 150 mM NaCl, 5 mM EDTA, 10% Glycerin) with protease inhibitor cocktail (B14001, Bimake) for 30 min at 4 C, followed by the enrichment of target proteins using Flag-M2 beads (B23102, Bimake) or specific antibodies overnight at 4 C

One may consider switching to antiretroviral agents with lower propensity for causing hyperlipidaemia provided that viral control is not jeopardised

Khaled Abd EA, Hanan N, Shwikar A, Ahmed E, Hany A, Doaa H

is supported by the Deutsche Forschungsgemeinschaft (grant CRC 1134, B02)