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Determination of antioxidant enzymes activities 1 g of leaf samples were freezed in liquid nitrogen followed by grinding in 10 mL extraction buffer (0.1 M phosphate buffer, pH 7.5, containing 0.5 mM EDTA in case of SOD, GPOX, CAT and 1 mM ascorbic acid in case of APX to prepare the extract

4, severe injury (Widespread loss of the brush border and tubular dilation in tubules, with cast formation commonly observed.)

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The aging process decreases the ability of cells to produce glutathione

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