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The cells were cultured as a monolayer in Dulbeccos modified Eagle medium (DMEM

An ideal temperature is -20C (-4F)

Gifford, D

Additionally, the diet reduces the expression of microglial activation markers (e.g., Iba1, CD11b), mitigating neuroinflammation, with its protective effects independent of amyloid- plaque reduction, thus offering new targets for intervening in early cognitive dysfunction (Di Lucente et al., 2024)