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Description

10.3389/fmed.2020.527059 137

Our experience shows that researchers who ignore this relationship are consistently frustrated with their lack of progress

Transfection, lentiviral transduction and secretion determination Transfection of DNA constructs into cells was performed using PEI (Polysciences, 23966) for HEK293T and X-tremeGENE HP (Roche, 6366244001) for U2OS according to the manufacturers protocols