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Glutathione is synthesized in the cytosol of each cell by the action of two enzymes in an elegantly regulated system that allows it to be maintained at different homeostatic levels in different tissue types, with the liver, given its detoxification role, having the highest amounts

After washing in PBS, slides were incubated in a 1:200 dilution of donkey polyclonal antibody to rabbit IgG Alexa Fluor (ab175692, Abcam) in blocking solution for 30 min

100 units = 1 mL

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