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MDA determination A 0.2 ml sample or standard was mixed well with 1 mL of 25 mM/L chromogen (thiobarbituric acid), covered, and heated in a boiling water bath for 30 min

(2019) 103:e1e13

The fish ( n = 160) were divided into four groups (G1, G2, G3, and G4), with the control (G1) fed a basal diet, while G2 was fed a basal diet supplemented with 75 g CPH/kg and was reared in freshwater (carbonate alkalinity of 1.4 mol/L, pH = 7.19)

Cysteine, due to its limited intracellular availability, is the rate-limiting precursor in GSH synthesis