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MicroRNA-124-3p promotes apoptosis and autophagy of glioma cells by down-regulating CREBRF

Caution: Copper-containing supplements may be unsuitable for people advised to limit copper

Harvested cells were resuspended in 20 mM HEPES pH 7.0, 500 mM NaCl, 25 mM imidazole (lysis buffer) supplemented with protease inhibitor cocktail and then lysed by a cell disruptor at 25 kpsi (Constant Systems)

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39 This technique was primarily developed and studied in emergency department settings, where rapid stabilization and discharge are a priority