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After experimental pulmonary metastasis, IGF1R-deficient mice exhibited reduced tumor implantation in both LLC and melanoma models, as similarly observed upon heterotopic syngeneic transplantation

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2.9 Glutathione content measurement Tissue samples (1.5 g) were ground in 2 mL 60 mmol L 1 sodium phosphate buffer (pH 7.0), and refrigerated centrifugation was performed at 12,000 g for 15 min, after which 100 L of supernatant was obtained