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Fork cleavage-religation cycle and active transcription mediate replication restart after fork stalling at co-transcriptional R-loops

The protein was eluted in three steps, first with 5 ml of elution buffer containing 50 mM imidazole (50 mM NaH 2 PO 4 , 300 mM NaCl and 50 mM imidazole, pH 8.0) and then with 5 ml of elution buffer containing 100 mM imidazole and finally with 5 ml of elution buffer containing 500 mM of imidazole

C., & Saadoun, S

Zhu R, Wang Y, Zhang L, Guo Q

Means Normally we humans are born in oxygen our body takes 21% oxygen through breath sometimes body creates oxygenation stress it means, those organs, which are responsible for the good functions of our body oxygen molecule damages them