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Mitochondria were incubated with 0 or 0.5 mM H 2 O 2 at room temperature for 20 min prior to assay start

If more than five days have passed, its typically best to skip that dose and pick back up on your regular schedule the following week

Transfected cells were grown at 37 C in 5% CO 2 in DMEM high glucose GlutaMAX (Gibco-Life Technologies) supplemented with 1% penicillin/streptomycin (Sigma Aldrich) and 5% of heat-inactivated fetal bovine serum (PAA, GE Healthcare Life Sciences) and selected with 0.2 mg

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Brain Dev (1992) 14(3):1704