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For cell scratch assay, HaCaT cells (13 1e6) or human keratinocyte primary cultures (5 1e5) were seeded in six/twelve-well plates with Dulbeccos modified Eagles medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA)

Mallozzi, C., Di Stasi, M

The recommended daily dose of glutathione is 250-500 mg, which is generally regarded as safe, with possible mild side effects like bloating or cramping