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An aliquot from the wells containing the most active LacAnc100 and LaccID clones was inoculated in 3 ml of YPD (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-glucose (Thermo Scientific, 410955000) and 25 g ml 1 chloramphenicol (Merck, C0378)) and incubated at 30 C and 225 rpm for 24 h

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L.ArajoA

RB1 is another upstream suppressor of ACSL4, whose loss can activate transcription factor E2F1 binding to the promoter of ACSL4 by increasing the transcriptional levels of E2F1 , thus promoting the occurrence of ferroptosis [139]

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