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mouse anti--actin, dilution 1:1000) were added for overnight incubation at 4 C and the antibody-bound proteins were developed with the enhanced chemiluminescence reagent coupled with horseradish peroxidase-conjugated secondary antibody

By April 2026, they took it out of Category 2 because its nominators withdrew their nominationsbut that doesn't mean it's now clear to use

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