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Given an inferior performance of individual biomarkers in diagnosing early PD, combination of multiple biomarkers may greatly improve the diagnostic efficacy

Cycling was performed using an Eppendorf Mastercycler (Hamburg, Germany), as follows: a pre-PCR step of a 5-min denaturation at 94C, followed by 30 cycles of denaturation at 94C for 30 sec, annealing for 30 sec and extension at 72C for 30 sec and a final 5-min incubation at 72C

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