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10.1186/s12974-019-1676-0 J

After fatty acid treatment, the organoids were incubated with 3.8 M BODIPY 493/503 (Life Technologies) for 30 min in culture medium at 37 C and then washed two times with PBS before being replaced with fresh culture medium containing DRAQ5 (Thermo Fisher) at 1:1500

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Because icebergs are made from fresh snow which fell hundreds or thousands of years ago before being quickly preserved as compacted ice, iceberg waters in their original state are free of modern pollutants and have the pH of clean precipitation