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Secondly, for isolation of potential ligninolytic bacteria, a series of tenfold serial dilutions were made by adding 1 ml of enriched soil suspension to 9 ml of sterile normal saline and vortexing the mixture followed by transferring 100 L aliquots of the produced dilutions to surface inoculation of minimal salt medium agar plates containing 1 g/L KL and 0.05 g/L nystatin (to inhibit fungal growth)