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Lyophilization removes water from the peptide under low temperature and vacuum conditions, transforming it into a stable powder

In addition to phosphoinositol 4,5-bisphosphate-dependent FGF-2 oligomerisation concomitant with membrane insertion (41,43), disassembly of membrane-inserted FGF-2 oligomers at the outer leaflet, mediated by cell-surface heparan sulphates, was recently reported (44)

Target gene expression levels were normalized by 18 S ribosomal RNA (18 S ribosomal N1), and relative gene expression was calculated using the 2e(-Ct) method 21

doi:10.1111/j.0954-6820.1968.tb02452.x