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RajaSankar S, et al

The analysis of neuron density further supports these findings, with the model group showing a marked reduction in neuron density (Figure 8C), reflecting the adverse effects of sleep deprivation on neuronal health

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Timelines vary, but improvement may be seen within a few months, depending on individual response

Following incubation with primary antibodies and rinsing, the blots were incubated for 1 h at room temperature with HRP-conjugated secondary antibody, according to the manufacturers recommended ratio