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Quantitative RT-PCR Total RNA was extracted using PureLink RNA isolation (Life Technologies, 12183025) and treated with DNase (Life Technologies, AM2222)

CCl 4 administration causes reactive metabolites and oxidative stress, resulting in hepatocellular necrosis and liver failure

J Am Soc Nephrol 25:978989

Research has demonstrated that the augmentation of NCOA4-mediated autophagic degradation of ferritin, commonly referred to as ferroautophagy, induces the accumulation of labile iron pool (LIP) and reactive oxygen species (ROS), ultimately leading to the occurrence of cell ferroptosis ( Research has substantiated that the expression of CACNA1G-AS1 is considerably elevated in ovarian cancer tissues and induces upregulation of FTH1 via IGF2BP1-mediated m6A methylation

Perfluorooctanoic acid (PFOA) has a damaging effect on the liver, and vitamin C supplementation can reduce PFOA-induced total cholesterol and triglyceride levels and reduce abnormal enlargement of the liver, mainly by inhibiting linoleic acid metabolism and increasing glutathione in the liver (128)