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Methylene blue (MB), also known as methylthioninium chloride, is a synthetic heterocyclic aromatic compound with the molecular formula CHClNS and a molecular weight of 319.85 g/mol (NIST Chemistry WebBook)

significantly enhanced fluorescence signals for MDA and 4-HNE measured in OA patient cartilage tissue by techniques like immunofluorescence staining (Shah et al., 2005)

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The applications in gene knockdown and overexpression experiments in K-562 cells dramatically enhanced the extent of gene alteration, with the gene knockdown efficiency increasing from 7% to 60% and the gene overexpression level rising from 47 to 253 times

Visser, M