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10 l from each well was acquired on a BD FACSCelesta, then the median fluorescence intensity (MFI) of the PE-positive cells was calculated for each well using FlowJo v10.8 Software (BD Life Sciences)

Based on the increase in cardiolipin biosynthesis, we hypothesize that luseogliflozin facilitates the maintenance of mitochondrial ATP production in response to I/R injury-induced tissue damage and oxidative stress

NNMT influences this regulatory landscape via: Reducing SAM availability for DNA and histone methylation Causing altered chromatin states that can activate oncogenes or silence tumor suppressors Facilitating oncogenic reprogramming in both tumor cells and stromal microenvironment 9

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