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A biosynthetic route to dehydroalanine-containing proteins

SuperScript VILO Master Mix (Invitrogen) was used to synthesize cDNA with 1 g of RNA per 20 l reaction

In contrast, Walia et al

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Activated cisplatin preferentially binds to purine bases in the nucleophilic N7 sites, where the majority of adducts occur between two guanines on the same strand, whereas ~35% of cisplatin adducts react with purines at the opposite strands, forming interstrand crosslinks (ICLs)