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After 2-3 days the cells enter the blast stage (cell division) where they increase considerably in size and their morphology changes to a more stretched appearance

HeLa and HEK-293T cells were maintained in either DMEM media (Gibco) containing 4.5g/L glucose, 110mg/L pyruvate, 4mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin or in RPMI media (Gibco) containing 2 mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin

Oxidation Sensitivity: Vitamin C is highly susceptible to oxidation when exposed to air, light, or elevated temperatures

The evolution from drug-sensitive to insensitive cells does not fall into the emergence category as it reflects rescuing mutations in a few cells that take over and regenerate a whole population [246]