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Total protein was extracted with a combination of RIPA buffer (Sigma Aldrich, US) and a protease inhibitor cocktail (Roche, Shanghai, CN) per the manufacturers instructions

Australias TGA Medical Devices The Therapeutic Goods Administration (TGA) is the unit of the Australian governments Department of Health and Aging that oversees medical device regulation in Australia

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Preclinical evaluation of the antineoplastic action of 5-aza-2-deoxycytidine and different histone deacetylase inhibitors on human Ewings sarcoma cells