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3.4 Inhibition of intracellular synthesis of reduced glutathione does not impair its extracellular action In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of -glutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 6)

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In addition, Zous group reported the induction of tumor cell ferroptosis due to the suppression of the GSH/GPX4 axis by CD8 + T cellmediated IFN- upon ICB (48), confirming a generalizable ferroptotic pathway also used by tumor cells for survival (6)