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Furthermore, reducing the amount of methanol in the solvent system significantly slows down the formation of 5HP2O on the AON, as shown by HPLC experiments

In recent years, MFH species have achieved positive results in the fields of diabetes (19), oncology (20), Alzheimers disease (21) and immunomodulation (22), reflecting their growing research interest in age-related conditions across different physiological systems

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The cells were then incubated for an additional 24 h and 48 h