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Following permeabilization (0.2% Triton-X100 in PBS, 10 min, RT), endogenous peroxidases were blocked by a 10 min incubation in a 3% H 2 O 2 solution, followed by a 1 h RT incubation in PBS with 2% bovine serum albumin (BSA, Amresco, Solon, OH)