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The covalent NLRP3-inflammasome inhibitor Oridonin relieves myocardial infarction induced myocardial fibrosis and cardiac remodeling in mice

A80-229AP, Bethyl Laboratories, Inc.), diluted in PBS/T/M (pH 5.5 or pH 7.4), was added for 1 h at room temperature, followed by a washing step and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)

Each method has its place

This isn't just correlationthe peptide appears to actively reset cellular programming in ways that promote longevity and health span