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ADP-ribose-specific chromatin-affinity purification for investigating genome-wide or locus-specific chromatin ADP-ribosylation

In Neurospora crassa , molecular analyses delineated distinct anabolic functions for NADP-GDH and catabolic functions for NAD-GDH, with the am gene encoding NADP-GDH extensively characterized at biochemical and genetic levels [54, 55]

The beads were collected and washed, and the bound proteins were eluted by Laemmli buffer containing 500 L 6 M urea, 25 L 200 mM DTT, and 25 L 500 mM IAA in the dark at 25C for 30 minutes

NADP reduction and glutathione oxidation, represented an opportunity to benchmark the recently introduced biosensor NERNST against NAPstars and roGFP2-Grx1 probes in vivo

Nuclei were stained with 5 g/mL DAPI