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Colony-forming efficiency Approximately 2 10 3 primary tendon cells were seeded into 25 T cell culture flasks (Corning), incubated with 10% FBS medium for 2 days, and their clonogenic capacity was determined in DMEM basal medium (2% FBS, 1% ITSE, 300 g/ml l-glutamine, 1% antibiotic-antimicotic solutions), supplemented with 10 M 29-mer, 34-mer, or peptide solvent (DMSO

2 L-serine and metabolic regulation 2.1 L-serine forms a complex metabolic network As a glycogenic amino acid, L-serine has various sources, such as an external diet, intracellular synthesis (from the glycolytic intermediate 3-phosphoglycerate and glycine), and cleavage of proteins and phospholipids in vivo ( in situ ( de novo synthesis, L-serine is converted from 3-phosphoglycerate (3-PG), an intermediate of glycolysis, through a three-step enzymatic reaction, in which 3-phosphoglycerate dehydrogenase (PHGDH) is the first rate-limiting enzyme ( Figure 1a ) ( Figure 1b ) ( Figure 1 Therefore, L-serine is a precursor molecule for the synthesis of many substances, and the metabolism of L-serine includes the synthesis of amino acids, phospholipids, and proteins ( Figure 1c ) ( Figure 1d ) ( Figure 1

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Zheng, P., Schramm, R