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doi: 10.1111/ejn.14163 163

Su D, Ding C, Wang R, Qiu J, Liu Y, Tao J, et al

After the addition of 100 l of 114 mm-acetic acid and incubation at room temperature for 5 min, readings were obtained again and absorbances defined as A 2 and B 2

Using mitragynine as a surrogate of the complex mixture, inhibition of intestinal and hepatic CYP3A (time-dependent and reversible) and hepatic CYP2D6 (reversible) by mitragynine was incorporated into the model based on in vitro observations