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Cells were incubated for 1 h at 37 C in a non-CO 2 incubator, with or without etomoxir (50 M)

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This M1-polarized microglia induce an inflammatory response and release a large number of inflammatory factors, such as nitric oxide, IL-6, TNF-, and reactive oxygen species (ROS), which are key drivers of hypothalamic inflammation (8689)

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After incubation, the cells were washed with PBS, and the GSH levels were quantified