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Confocal imaging An inverted LSM880 microscope was used with a Plan-Apochromat 63 1.4 [numerical aperture (NA)] oil objective with a working distance of 0.19 mm

PLoS Biol 16:e2006203 Friedmann Angeli JP, Schneider M, Proneth B, Tyurina YY, Tyurin VA, Hammond VJ, Herbach N, Aichler M, Walch A, Eggenhofer E, Basavarajappa D, Radmark O, Kobayashi S, Seibt T, Beck H, Neff F, Esposito I, Wanke R, Forster H, Yefremova O, Heinrichmeyer M, Bornkamm GW, Geissler EK, Thomas SB, Stockwell BR, ODonnell VB, Kagan VE, Schick JA, Conrad M (2014) Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice

Drink plenty of water for its health benefits like helping to prevent headaches and constipation, but also for its potential impact on weight loss itself and because thirst may be misinterpreted by the body as hunger

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