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Clean Vial Stoppers and Surfaces: Wipe the rubber stopper of the vial with 70% isopropanol or an alcohol swab before puncturing it with a needle

K., Malmstrm, J., Hjlys-Larsen, K

The synthesis method of the Cagrilintide comprises the third step of preparing 200mL of cracking reagent, adding the full-protection peptide under an ice bath condition, returning to room temperature after 0.5h, continuing to react for 2h, adding anhydrous ether for precipitation after the reaction is finished, performing centrifugal precipitation for multiple times, adding 300mL of ether each time, and drying to obtain the Cagrilintide product

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Based on these observations, we designed peptides in which the individual hydrophobic residues were substituted with alanine