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This is illustrated by the termination of the BEACON (NCT01351675, Bardoxolone Methyl Evaluation in Patients with Advanced Chronic Kidney Disease and Type 2 Diabetes, 2011) and CARDINAL (NCT03019185, Patients with Alport Syndrome, 2017) trials due to cardiovascular safety concerns, despite achieving renal benefit-highlighting the imperative for rigorous long-term safety evaluation

2011;7:539 Kelley LA, Mezulis S, Yates CM, Wass MN, Sternberg MJ

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For collagen cultures, ileum organoids grown in BME were first released from the matrix by incubation with dispase (10 U ml 1 for 30 min at 37 C)

& Dogan, I